Figure 1

Figure 1

HIVEP1 mRNA expression is increased in patients with sepsis and upon stimulation with bacterial agonists in vitro. HIVEP1 mRNA in PBMCs (A) and monocytes (B) from patients with sepsis (5 and 8 individual donors, respectively) upon admission to the intensive care unit and in healthy subjects (5 and 6 individual donors, respectively). The monocytes data was obtained from GEO database (GSE46955). (C) HIVEP1 mRNA in human monocytic wild type THP1-MD2-CD14 cells incubated with medium, PAM3CSK4 (1 µg/ml) or LPS (100 ng/mL) for 4 hours. (D) HIVEP1 mRNA in primary human monocytes incubated with medium (control), PAM3CSK4 (1 µg/ml) or LPS (100 ng/mL) for 4 hours. For (C), three experiments with four replicates were conducted. For (D), two experiments each with 4 independent donors (8 individual donors in total) were conducted. Pooled data from three (C) or two (D) independent experiments are displayed. (E) HIVEP1 mRNA in primary human monocytes (from 4 individual donors) incubated with medium or LPS (100 ng/mL) for 2 hours in the presence or absence of the NFκB inhibitor BAY11-7082 (or vehicle) added 30 minutes prior to LPS. HIVEP1 mRNA expression was normalized to HPRT mRNA (A, C, D, E). For (B) normalized gene expression values were used as provided in GSE46955. Data shows means ± SEM with individual data shown as dots. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.