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Figure 6
Overexpression of miR-202-5p partially rescues PGC migration in embryos overexpressing rab10. (A–F) Representative images of PGCs in embryos injected with 200 pg rfp mRNA and 300 pg control siRNA or control mimics (A,D), 100 pg rab10 mRNA and 300 pg control siRNA or control mimics (B,E), 100 pg rab10 mRNA and 300 pg rab10 siRNA or miR-202-5p mimics (C,F). Arrowheads indicate mislocalized PGCs. The corresponding phenotypes in different groups of embryos were shown in the upper right of the panels (A–F). (G) The percentage of embryos with mislocalized PGCs in (A–F). (H) The number of mislocalized PGCs in each embryo in (A–F). (G,H) The results were representative of more than three independent experiments in triplicate. The average number of mislocalized PGCs were showed in red line by mean ± sd. *** p < 0.001, **** p < 0.0001. Scale bar, 100 µm.