ZFIN Image: Gui et al., 2021, Figure 3.

Image

Figure Caption

Figure 3.

Tracheal DMTs contain a luminal bundle of tektin filaments

(A) Cross-section of the bovine DMT, showing a luminal bundle of tektin filaments. The luminal-most tektin filaments (circled) are absent from some particles. The MIPs and PF A12 that interact with tektins are labeled.

(B) Interaction network showing the pentagonal organization of tektin paralogs and TEKTIP1. Intradimer contacts are shown in red, and interdimer contacts are shown in black.

(C) Secondary structure profile (top) and tertiary structure (bottom) of a tektin 1 monomer.

(D) Superposition of tektin paralogs. The conserved rod domains superpose with a root-mean-square deviation (RMSD) of 1.0–1.7 Å.

(E) Quaternary structure of a tektin filament. The expanded view shows the L12 loop clamping around the L2 loop of an adjacent molecule.

(F) Interactions between tektin dimers as they occur in a section of the 8-tektin bundle. Filament orientation is indicated by labeling their N and C termini. Arrows mark the start of helix 1A in each tektin molecule to show the offsets in their registry. The two T2:T4 heterodimers are identical except that their N and C termini adopt different conformations because of the interactions they make within the bundle.

See also Figures S3 and S4, Table S1, and Video S1.

Acknowledgments

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Reprinted from Cell, 184(23), Gui, M., Farley, H., Anujan, P., Anderson, J.R., Maxwell, D.W., Whitchurch, J.B., Botsch, J.J., Qiu, T., Meleppattu, S., Singh, S.K., Zhang, Q., Thompson, J., Lucas, J.S., Bingle, C.D., Norris, D.P., Roy, S., Brown, A., De novo identification of mammalian ciliary motility proteins using cryo-EM, 5791-5806.e19, Copyright (2021) with permission from Elsevier. Full text @ Cell