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Fig. 6 (A,B) All wild-type uninjured and 3 dpci Tg(BAC-runx1P2:Citrine;kdrl:Hsa.HRASmCherry) double-positive cells visualised in an UMAP plot. (A) Expression of collagen 1a1b specifically in 3 dpci double-positive cells. (B) Cell clustering within the double-positive population identifies six different cell clusters. (C) Heatmap showing that both clusters 4 and 5 express high levels of collagens, whereas cluster 4 specifically expresses smooth muscle genes. (D) myh11a and tagln are expressed in cluster 4 after injury. Arrowhead indicates myh11a expression in cell cluster 4. (E-F) Immunohistochemistry for Citrine, mCherry and Myh11. Arrowheads indicate expression of Myh11 in Tg(BAC-runx1P2:Citrine;kdrl:Hsa.HRAS-mCherry) double-positive cells at 3 (E,E′) and 7 (F) dpci. en, endocardium; v, ventricle; w, wound. Scale bars: 100 μm.