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Fig. 4 tnnc2 and tnni4b.3 reporter expression in the adult zebrafish heart. (A) Sagittal section of a Tg(tnnc2:EGFP) adult heart stained for EGFP expression (green), HABP (red) and DNA (blue). Cyan arrowheads point to tnnc2:EGFP+ CMs in the atrioventricular (AV) and bulboventricular (BV) boundaries. Insets show high magnification images of the ventricular wall. Yellow arrowheads point to tnnc2:EGFP+ primordial CMs. Magenta arrowheads point to HABP staining. White dashed line outlines the heart. (B) tnnc2:EGFP (green line) and HABP (red line) fluorescence intensity measured along the white dotted line in (A). (C) Schematic representation of tnnc2:EGFP+ CMs and Hyaluronan rich connective tissue in the adult zebrafish heart. (D) Sagittal section of a Tg(tnnc2:EGFP) adult ventricular wall stained for EGFP expression (green), α-Actinin (white), and DNA (blue). Magenta arrowheads point to tnnc2:EGFP+ primordial layer CMs. Yellow arrowheads point to tnnc2:EGFP+ CMs lacking α-Actinin striations. (E) Quantification of α-Actinin fluorescence intensity on a 10 μm line drawn along cortical/primordial/trabecular CMs perpendicular to the striations. (F) Unstained sagittal section of a Tg(tnni4b.3:EGFP); Tg(myl7:DsRed2-NLS) adult zebrafish heart. Tr: trabecular myocardium; At: atrium; Ba: bulbus arteriosus. Scale bars: 100 μm (A, F) and 10 μm (D).
Reprinted from Developmental Biology, 476, Tsedeke, A.T., Allanki, S., Gentile, A., Jimenez-Amilburu, V., Rasouli, S.J., Guenther, S., Lai, S.L., Stainier, D.Y.R., Marín-Juez, R., Cardiomyocyte heterogeneity during zebrafish development and regeneration, 259-271, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.