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Figure 3
Role of AfLkhA in gliotoxin production. (A) Determination of GT production in WT, ΔAflkhA, and C’AflkhA strains. GT were extracted with chloroform from culture supernatant of each strain and subjected to TLC. Std: GT standard (Simga-Aldrich). (B–E) Total RNA was extracted from mycelial balls incubated in Czapek Dox broth. RT-qPCR analysis was performed using 18S rRNA gene as an internal control. Expression patterns of GT cluster genes, transcription factor gliZ (B), nonribosomal peptide synthetase gliP (C), transporter gliA (D), and transcriptional activator zafA (E). ***P < 0.001.
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Full text @ Front Cell Infect Microbiol