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Figure 1
Vegetative growth and asexual development of ?AflkhA strain. (A) Colony morphology of WT, ?AflkhA, and C?AflkhA strains. Spores were inoculated onto GMM agar and incubated at 37°C for two days. (B) Radial growth of WT, ?AflkhA, and C?AflkhA strains. Colony diameter of each strain was measured daily from point of inoculation over five days. When error bars are not shown, they are smaller than symbols (n = 5). (C) Mycelial production on liquid YCGMM. Dry weight of each strain was measured. When error bars are not shown, they are smaller than symbols (n = 3). (D) Conidiophore heads on GMM agar. (E) Quantification of conidia production (n = 4). *P < 0.05. (F-H) Expression patterns of brlA (F), abaA (G) and vosA (H) genes. Mycelial balls of strains obtained from YCGMM liquid culture were transferred to GMM agar and incubated for indicated hrs. Total RNA was extracted and RT-qPCR analysis was performed using 18S rRNA gene as an internal control. **P < 0.01, ***P < 0.001.