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Figure 4

ID: ZDB-IMAGE-211103-29
Publication: Kler et al., 2021 - Cre-Dependent Anterograde Transsynaptic Labeling and Functional Imaging in Zebrafish Using VSV With Reduced Cytotoxicity
All Figures: Figures for Kler et al., 2021

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Figure Caption

Figure 4

Temporal dynamic of calcium activities in multiple conditions captured from the neurons infected with VSV(M51R)ΔG-Cre in gad1b:LRL-Gal4;UAS:GCaMP6s fish. All images shown are confocal optical sections. (A–A”) Neurons (A’—Mesencephalon neuron and A”—Dorsal Thalamus neuron) expressing GCaMP6s detected in one XY plain of the brain in a 3 dpi (6 dpf) fish. (B) The evoked transient calcium activity traces (F_t − F₀/F₀) during the whole-field display of moving gratings. (C) The evoked transient calcium activity traces during whole field display of 18-s dark/2-s bright flashes. Scale bar in (A) is 100 μm. Scale bars in (A’,A”) are 25 μm.

Acknowledgments

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