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Figure 5
Effect of estradiol on 6-OHDA-induced locomotor deficit in the zebrafish PD model. Zebrafish were pretreated with 10, 100, and 1000 nM E2 from 9 hpf to 5 dpf and then challenged with 250 μM 6-OHDA from 2 to 5 dpf. The locomotor behavior was measured at 5, 6, and 7 dpf of the control, 6-OHDA, 6-OHDA plus 10 nM E2, 6-OHDA plus 100 nM E2, 6-OHDA plus 1000 nM E2, 10 nM E2, 100 nM E2, and 1000 nM E2 groups. The upper panel demonstrates a representative swimming pattern, and the lower panel shows the average total swimming distance. The value corresponds to the mean ± SEM of two independent experiments that have been done in octuplicate (n = 16). ∗p < 0.05 compared with the control group; #p < 0.05 compared with the 6-OHDA group.