|
Figure 4
Effect of lithium chloride and estradiol on growth associated protein 43 (GAP-43), N-myc, and TGase in SH-SY5Y cells. (a) SH-SY5Y cells were co-treated with 1 mM LiCl in the absence or presence of 100 nM E2 for 24 hr. Western blotting for GAP-43 and N-myc of the control, 1 mM LiCl, 100 nM E2 and 1 mM LiCl plus 100 nM E2 groups. (b) The quantification result of GAP-43 relative density is shown. (c) The quantification result of TGase relative density is shown. (d) The quantification results of N-myc relative density are shown. β-Actin was used as an internal control for normalization. The value corresponds to the mean ± SEM of three independent experiments that have been done in triplicate (n = 9). ∗p < 0.05 compared with the control group; #p < 0.05 compared with the 6-OHDA group.