Figure 6
Degradation of DHSATD (XI in Figure 1, closed squares) and formation of MDTETD (XIII, open squares) by suspensions of cholate-grown cells of Sphingobium sp. strain Chol11 (initial OD600 = 0.13) under oxic (A) and anoxic (B) conditions. (C) HPLC-MS analyses of supernatants after 31 h of oxic (top) and anoxic (bottom) incubation. MS base peak chromatograms measured in negative mode are shown. (D) Proposed structure for a steroid compound named XIV found in cell suspensions incubated anoxically with DHSATD and comparison of characteristics of DHSATD and XIV. The structure suggestion is based on m/z values and absorption spectra. Error bars indicate standard deviation, which may not be visible if too small (n = 3).