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Figure 5
Knockdown of GrnA decreased the expression of HGF/c-met axis in hepatectomized zebrafish. Quantitative PCR analysis of zebrafish HGF paralogs, (A) HGFa and (B) HGFb, as well as the HGF-specific receptor, (C) c-met, in control and GrnA morphant zebrafish after PHx. The relative expression levels compared to control group at 0 h after PHx are presented as mean ± SD. EF1a served as the internal control. (D) Protein levels of p-met, HGF, and GAPDH were examined by Western blot analysis at 48 h after PHx. The levels of p-met and HGF proteins were normalized to GAPDH protein levels and then compared to control group. The fold change compared to controls is presented as mean ± SD. (E) Quantitative PCR analysis of c-met upstream signaling-related genes, including MMP2, MIF, and YBX1, and c-met downstream signaling-related genes, including Sub1, Rac1, rps6kb1, and Fn1. The relative expression levels compared to control group are presented as mean ± SD. EF1a served as the internal control. Significance was set at * p < 0.05, ** p < 0.01, *** p < 0.001, as determined by t-test; PHx, partial hepatectomy; p-met, phospho-met.