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Figure 4
Treatment with BLD-2736 increased the activity of the autophagy protein quality control pathway. (A) Immunoblotting of LC3II revealed changes in the levels of LC3II relative to GAPDH across treatment groups. (B) Quantitative analysis of the ratio of LC3II to GAPDH was found to be significantly increased in larvae treated with 300 nM BLD-2736 when compared to larvae treated with the autophagy inhibitor chloroquine and 300 nM BLD-2736, indicating increased synthesis of LC3II and induction of autophagy. Protein lysates were produced from pooled samples of n = 12–20 zebrafish larvae. Each data point represents an independent experimental replicate. All data are presented as mean ± SEM. * represents p < 0.05, ** represents p < 0.01, **** represents p < 0.0001.