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Figure 3 IF of SJD.1 after creating scratches in the confluent cells and treating them with CRTAC1 proteins (dlCrtac1a, dlCrtac1b and hCRTAC1-A) compared with the control cells. Colocalization of ?-tubulin (green-fluorescence), F-actin (red fluorescence) and DAPI (nuclei, blue fluorescence). Images are representative of control cells, and CRTAC1 treated cells at 6 h and 24 h after scratching the confluent cell layer. Images were obtained with a Leica DM IL microscope coupled to a Visicam PRO 20C digital camera and photographs were analyzed using ImageJ software for image overlay. Scale bars indicate 100 ?m.