IMAGE

Figure 1

ID
ZDB-IMAGE-211025-124
Source
Figures for Tanoue et al., 2021
Image
Figure Caption

Figure 1

Generation of podocyte-specific Crb2 knockout mice. (a) Two loxP sites were inserted into introns 6 and 8 of the Crb2 gene to skip exons 7 and 8 in the Crb2 floxed allele. (b) While there was a 353-bp band with Crb2 primers in Crb2fl/fl mice and Crb2fl/flpod-CreTg/+ mice, there was a 153-bp product in Crb2+/+pod-CreTg/+ mice. There was a 102-bp band with pod-Cre primers in Crb2+/+pod-CreTg/+ mice and Crb2fl/flpod-CreTg/+ mice. (c) There were a strong band between 140–180 kDa and a weak band between 180–245 kDa markers with CRB2 antibody in the glomeruli from Crb2+/+pod-CreTg/+ mice or Crb2fl/fl mice, while these bands were invisible in those of Crb2fl/flpod-CreTg/+ mice at 2 months of age. The band of β-actin was similar among the three groups. (d) Synpo, a podocyte marker, was co-localized with Crb2 in Crb2+/+pod-CreTg/+ or Crb2fl/fl mice. No co-localization of Crb2 and Synpo was observed in Crb2fl/flpod-CreTg/+ mice. Scale bars 20 μm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.