Figure 3

Figure 3

Effects of GD intake on ICR mice on a high-fat diet (HFD). Change in plasma TG (A), plasma TCHO (B), and VAT volume (C) after 2 weeks of GD administration. (D) Represents micro-CT images of the four groups. The red dotted lines demarcate the area for which VAT volume was measured (from the xiphoid process of the sternum to the hip joint). Yellow indicates the visceral adipose tissue, and orange indicates the subcutaneous adipose tissue. (E) GD-induced Ucp1 expression in mice eWAT. (F) Fluorescent immunohistochemical staining (FIHC) with anti-mouse Ucp1 antibody in eWAT sections. PD, phrase difference images; Scale bar = 50 μm. (G) Average adipocyte size (n = 30) of the four mouse groups in (F). *p < 0.05, **p < 0.01 vs. HFD, n = 10, error bars indicate SD.