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Fig. 2 Adult color pattern formation in A. ocellaris is linked to a switch in expression of pigment cells–specific genes during postembryonic development. (A and B) Stereomicroscope images of entire larvae and the associated zoom of the trunk at stage 1 (A), 2 (B), 3 (C), 4 (D), 5 (E), 6 (F), and 7 (G) (adapted from ref. 25). The white and red arrowheads point to white bars and black stripes and black and orange arrows point respectively to melanophores and xanthophores. (H and I) Principal component analysis (PCA) analysis of the pigmentation genes (H) and iridophores genes (I) expression from transcriptomic analysis from entire larvae over postembryonic stages. The two PCA exhibit a clear separation between stages 1 to 3 and stages 4 to 7. The ellipses were arbitrarily drawn around arrays to help resolution: stages 1 to 3 (orange) and 4 to 7 (blue) arrays. All stages had 3 replicates. (J) Heatmap of the seven iridophore genes having the highest fold change between stages 1 to 3 and stages 5 to 7. The color represents the intensity of the centered (but unscaled) signal that goes, for each gene, from low (blue) to medium (white) to high (red).