Fig. 2

Fig. 2

a Schematic of the melanoma tumor transplantation assay. b, c Transplanted nf1/pten-mutant melanoma tumor cells were monitored daily in 3-week-old rag2^−/− recipient zebrafish treated with DMSO (CTR; n = 12), 80 nM trametinib (n = 11), 2 μM buparlisib (n = 11), or the combination of 80 nM trametinib and 2 μM buparlisib (n = 12) for 6 days. Kaplan–Meier curves for progression-free survival (PFS, b) and overall survival (OS, c) are shown. Statistical analyses were performed by log-rank test, comparing drug-treated with DMSO-treated zebrafish. d, e Transplanted nf1/pten-mutant melanoma tumor cells were monitored daily in 3-week-old rag2^−/− recipient zebrafish treated with DMSO (CTR; n = 12, same values as in b, c), 20 μM sirolimus (n = 12), 20 μM everolimus (n = 11) or 40 μM temsirolimus (n = 11) for 6 days. Kaplan–Meier curves are shown, with statistical analyses performed as in b, c. For all experiments involving drug treatments, drugs were replenished every 2 days during the 6-day course of treatment (black arrows).