Figure 8

Figure 8

In vitro ECs migration on collagen I and collagen IV substrates. The blue region denotes the scratch wound mask over time (0 h, 4 h, 8 h, and 12 h). ECs migrate into the wound area on non-coated (a), collagen I-coated (b) and collagen IV-coated wells (c). The red area depicts the wound closure by EC expansion over time. The most effective and fast EC migration is observed with the collagen I substrate, followed by collagen IV and lastly, the least efficient, non-coated wells at 0, 4, 8, and 12 h (a–c’’’; d, e, and f). Relative wound density (d), wound confluence (e) and wound width (f) has been quantified. Cells in the wound area are the most confluent in collagen I-coated wells, followed by collagen IV and non-coated wells (e). Blue dashed line - collagen I, green dashed line - collagen IV, purple dashed line - no collagen, black dashed line - time points (4 h, 8 h, 12 h).