FIGURE 1

FIGURE 1

SWS-derived fibroblasts show altered HSPGs and glypicans after heparinase III (H’ase III) digestion. (A) Western blotting of ΔHS-stub using 3G10 antibody following heparinase III digestion of three SWS-derived fibroblasts and two control fibroblasts. C1 and C2 are control fibroblasts. C1, GM08429; C2, GM08680. P1.1, P4.1, and P5.1 are SWS-derived fibroblasts. (B) Quantitation assay of glypican band density in (A) and two other replicates. The data are presented as mean ± SEM. An unpaired two-tailed t-test was used. ^*⁣*⁣**p < 0.0001. (C) Agarose gel of six human glypicans after reverse-transcript PCR. (D) qPCR of three dominant glypicans in SWS-derived fibroblasts. The relative glypican level was normalized to GAPDH. The graphs represent the 2^–ΔΔCt values. Experiments were performed in triplicates with similar results.