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Figure 7
Effect of ?-lipoic acid on CRS-3. (A?F) The heart rate, blood flow, heart dilatation and survival rate of ARF larvae treated with ?-lipoic acid (4 ?M, 8 ?M) and the untreated group are presented. The number of larvae in each group was five, except for the survival rate group (n = 60). Scale bar: 100 ?m. (G,H) MTZ-induced ROS in zebrafish larvae (n = 5) can be attenuated by ?-lipoic acid. The image shows that ROS significantly decreased as the drug concentration increased. The white arrow points to the heart area. The Scale bar of (B,G) are 100 ?m and 200 ?m, respectively. Mean ± SEM; ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001, ANOVA/Dunnett?s test compared with untreated ARF larvae.