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Figure 4
ARF induced heart failure in zebrafish larvae. The heart rate (A?C) and heart size (D) of Tg(cdh17:Dendra2-NTR;cmlc2:GFP) zebrafish larvae treated with MTZ or control were detected (n = 5). (E) TUNEL-positive apoptotic cardiomyocytes (red) were detected in the heart of Tg(cdh17:Dendra2-NTR;cmlc2:GFP) zebrafish larvae treated with MTZ, but not in the control (n = 30). Scare bar: 100 ?m. Mean ± SEM; ns, not significant; ** p < 0.01, *** p < 0.001, **** p < 0.0001, ANOVA/Dunnett?s test compared with control.
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