Fig. 8.

Fig. 8.

JAK inhibition impairs normal mitochondrial transcription and cell proliferation in the intestine of 6 dpf larvae. (A) WISH with anti-mt_nd2 mRNA probe on 6 dpf larvae treated with 60 µM AG490 from 24 to 72 hpf and DMSO-treated controls; zoom on the intestine. (B) Quantification of mt_nd2 mRNA expression in the intestine (n=30). (C) Phospho-Histone-H3 (pH3) immunostaining of 6 dpf AG490-treated larvae and DMSO-treated controls; inset shows magnification of boxed area of intestine. Arrowheads indicate pH3-positive cells. (D) Quantification of the number of AG490- and DMSO-treated larvae displaying intestinal proliferation (n=15). (E) AG490-treated larvae showing loss of folding in intestinal mucosa. (F) Graph showing the dimension of mucosal thickness in both DMSO- and AG490-treated 6 dpf larvae (n=18). Data are mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001 (unpaired two-tailed t-test on indicated number of samples). Scale bars: 200 μm in A,C; 100 μm in E.