ZFIN Image: Peron et al., 2021, Fig. 7.

Image

Figure Caption

Fig. 7.

JAK inhibition impairs normal mitochondrial transcription and cell proliferation in the TeO of 72 hpf embryos. (A) WISH with anti-mt_nd2 mRNA probe on 72 hpf embryos treated with 100 µM AG490 from 24-72 hpf and DMSO-treated controls. (B) Relative mt_nd2 transcript expression assayed by qRT-PCR in 48 and 72 hpf embryos treated with 100 µM AG490 and DMSO-treated controls starting from 24 hpf; zgapdh was used as internal control (P-values=0.6261; 0.0060). (C) FISH with anti-pcna probe in the TeO of 72 hpf embryos treated with 100 µM AG490 from 24 to 72 hpf and DMSO-treated controls. (D) Fluorescence quantification of pcna mRNA expression in the TeO (n=6) (P-value=0.0003). Data are mean±s.e.m. **P<0.01; ***P<0.001 (unpaired two-tailed t-test on three independent biological samples, where n not specified). ns, not significant. Scale bars: 200 μm in A; 100 μm in C.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development