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Fig. 7.

ID: ZDB-IMAGE-210930-26
Publication: Peron et al., 2021 - Y705 and S727 are required for mitochondrial import and transcriptional activities of STAT3 and regulate proliferation of embryonic and tissue stem cells
All Figures: Figures for Peron et al., 2021

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Figure Caption

Fig. 7.

JAK inhibition impairs normal mitochondrial transcription and cell proliferation in the TeO of 72 hpf embryos. (A) WISH with anti-mt_nd2 mRNA probe on 72 hpf embryos treated with 100 µM AG490 from 24-72 hpf and DMSO-treated controls. (B) Relative mt_nd2 transcript expression assayed by qRT-PCR in 48 and 72 hpf embryos treated with 100 µM AG490 and DMSO-treated controls starting from 24 hpf; zgapdh was used as internal control (P-values=0.6261; 0.0060). (C) FISH with anti-pcna probe in the TeO of 72 hpf embryos treated with 100 µM AG490 from 24 to 72 hpf and DMSO-treated controls. (D) Fluorescence quantification of pcna mRNA expression in the TeO (n=6) (P-value=0.0003). Data are mean±s.e.m. **P<0.01; ***P<0.001 (unpaired two-tailed t-test on three independent biological samples, where n not specified). ns, not significant. Scale bars: 200 μm in A; 100 μm in C.

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