Fig. 6.

Fig. 6.

mitoSTAT3-dependent activation of cell proliferation in the TeO depends on S727 phosphorylation. (A,A′) Representative images of WISH performed with an anti-pcna probe on 48 hpf uninjected larvae, larvae injected with MLS_mStat3_NES or MLS_mStat3_NES S727A mRNAs (A). Fluorescence quantification of pcna mRNA expression in the TeO (n=12) (A′). (B) qRT-PCR analysis of mt_nd2 in uninjected 48 hpf larvae and larvae injected with MLS_mStat3_NES mRNA treated or untreated with PD98059 for 24 h. (C) qRT-PCR analysis of pcna in uninjected 48 hpf larvae and larvae injected with MLS_mStat3_NES mRNA treated either with PD98059 12.5 µM or DMSO for 24 h. (D) qRT-PCR analysis of mt_nd2 in uninjected 48-hpf larvae and larvae injected with MLS_mStat3_NES S727D mRNA treated or untreated with PD98059 for 24 h. actb1 was used as an internal control. Data are mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001 (unpaired two-tailed t-test on three independent biological samples, where n not specified). ns, not significant. Scale bar: 100 μm.