Figure 3

Figure 3

Down‐regulated insulin receptor signaling pathway and downstream pathways in akr1a1a^−/− zebrafish larvae. A) Experimental design for larval RNA‐Seq. 30 larvae per clutch, 6 clutches of akr1a1a^+/+ and akr1a1a^−/− zebrafish larvae at 5dpf were applied for RNA isolation. B) Bubble plot showed the significantly down‐regulated biological pathways in akr1a1a^−/− zebrafish larvae at 5dpf via KEGG and GOBP analysis. Heatmaps showed relative mRNA expression in C) insulin receptor signaling pathway, D) MAPK, E) signal transduction by protein phosphorylation, and F) transmembrane receptor protein tyrosine kinase signaling pathway was down‐regulated significantly in akr1a1a^−/− zebrafish larvae. Higher and lower expression is displayed in red and blue, respectively. GSEA, gene set enrichment analysis. dpf, day post fertilization. KEGG, Kyoto encyclopedia of gene and genomes. GOBP, Gene ontology biological processes.