ZFIN Image: Neal et al., 2021, Fig. 6

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Figure Caption

Fig. 6

Intradermal injection of Ad-VEGFA164 results in sustained arterial and angiogenic enhancer activity, but venous enhancers were not reactivated. Ad-VEGFA164 was injected intradermally into the ears of adult Foxn1−/− mice transgenic for arterial and angiogenic-expressed Dll4in3:lacZ (A), angiogenic expressed HLX-3:lacZ (B), and venous-expressed Ephb4-2:lacZ (C) and CoupTFII-965:lacZ (D). Enhancer activity was assessed at the stated days after injection by X-gal staining and compared with uninjected control. Red arrowhead = artery, black arrowhead = blood vessel. N numbers are indicated on images in bottom right corner, represented as number of ears similar to image shown/total number of ears investigated. Examples of the alternative expression patterns can be seen in Fig. S11.

Acknowledgments

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Reprinted from Developmental Biology, 473, Neal, A., Nornes, S., Louphrasitthiphol, P., Sacilotto, N., Preston, M.D., Fleisinger, L., Payne, S., De Val, S., ETS factors are required but not sufficient for specific patterns of enhancer activity in different endothelial subtypes, 1-14, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.