ZFIN Image: Neal et al., 2021, Fig. 3

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Figure Caption

Fig. 3

Knockdown of erg and fli1 in zebrafish reduces activity of both venous and arterial enhancers, and reduces the endogenous expression of both venous and arterial genes. A-B. Representative tg(Ephb4-2WT:GFP) transgenic zebrafish (A), and wildtype zebrafish after whole-mount in situ hybridization for venous markers ephb4 and stab1l (B) after morpholino-induced erg/fli1 knockdown. C-D. Representative tg(Dll4in3WT:GFP) transgenic zebrafish (C), and wildtype zebrafish after whole-mount in situ hybridization for arterial markers dll4a and efnb2 (D) after morpholino-induced erg/fli1 knockdown. Numbers on top right of B and D indicate number of embryos with the predominant and displayed phenotype per total number of embryos analyzed. E-F. Graphs depicting observed GFP/endogenous gene expression levels. Ephb4-2:GFP cnt n = 460, 3 ng MO n = 420, 4.5 ng MO n = 279, 6 ng MO n = 341. Dll4in3:GFP cnt n = 34, 3 ng MO n = 29, 4.5 ng MO n = 67, 6 ng MO n = 27. ephb4 cnt n = 34, 6 ng MO n = 40; stab1l cnt n = 40, 6 ng MO n = 38; dll4a cnt n = 34, 6 ng MO n = 34; efnb2 cnt n = 38, 6 ng MO n = 38.

Acknowledgments

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Reprinted from Developmental Biology, 473, Neal, A., Nornes, S., Louphrasitthiphol, P., Sacilotto, N., Preston, M.D., Fleisinger, L., Payne, S., De Val, S., ETS factors are required but not sufficient for specific patterns of enhancer activity in different endothelial subtypes, 1-14, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.