ZFIN Image: Gurer et al., 2021, FIGURE 5

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FIGURE 5

Figure 5. DR5-AS-knockdown-mediated perturbations in gene expression. Total RNAs isolated from HeLa cells transfected with negative GapmeR or DR5-AS GapmeR were subjected to RNA-seq. A volcano plot and pie chart of differentially expressed genes after knockdown are presented in (A,B), respectively. (C) qPCR validation of some genes differentially expressed in DR5-AS knockdown HeLa cells. (D) Reactome Pathway analysis of genes differentially expressed in HeLa cells transfected with DR5-AS GapmeR. (E) qPCR analyses of immune system-related genes as determined to be differentially expressed based on the Reactome pathway analysis. ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001, and ^****p ≤ 0.0001.

Acknowledgments

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