FIGURE 1

FIGURE 1

Cisplatin-induced apoptosis and proliferation in HeLa cells. (A) Proliferation kinetics of HeLa cells. HeLa cells were treated with a range of cisplatin concentrations for 16 h and the proliferation rate was measured by WST-1 assay. (B) Percentage of live and early apoptotic population of HeLa cells. Hela cells were treated with 80 μM cisplatin (CP) for 16 h and the rate of apoptotic cells were determined by Annexin-V and 7AAD staining. The stained cells were analyzed by flow cytometry. DMSO (0.1%) was used as negative control. (C,D) The population distributions of DMSO control and CP-treated groups as flow cytometry dot blot graphs, respectively. ns, non-significant, p > 0.05, ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001, and ^****p ≤ 0.0001.