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Figure 4 Reduced proinflammatory myeloid cell populations and CD4 T cells in pancreatic lymph nodes of NOD:Alox15Δmyel mice. Pancreatic lymph nodes were isolated from NOD:Lyz2-Cre (Cre+) control mice and NOD:Alox15Δmyel (Δmyel) mice at 8 weeks of age and subjected to flow cytometry analysis. (A) Representative contour plot showing gating of F4/80+ TNF-α+ cells (left), total number of F4/80+ TNF-α+ cells (middle), and F4/80+ TNF-α+ cells as a percentage of total cells (right; n = 3–4 mice per genotype; *P < 0.05 by unpaired 2-tailed t test). (B) Representative contour plot showing gating of CD11c+ TNF-α+ cells (left), total number of CD11c+ TNF-α+ cells (middle), and CD11c+ TNF-α+ cells as a percentage of total cells (right; n = 3–4 mice per genotype; *P < 0.05 by unpaired 2-tailed t test). (C) Representative contour plot showing gating of CD4+ cells (left), total number of CD4+ cells (middle), and CD4+ cells as a percentage of total cells (right; n = 3–4 mice per genotype; *P < 0.05 by unpaired 2-tailed t test). All data are presented as mean ± SEM.