IMAGE

Figure 3

ID: ZDB-IMAGE-210908-11
Publication: Shimizu et al., 2021 - Glutamate 73 Promotes Anti-arrhythmic Effects of Voltage-Dependent Anion Channel Through Regulation of Mitochondrial Ca²⁺ Uptake
All Figures: Figures for Shimizu et al., 2021

Image

Figure Caption

Figure 3

Overexpression of VDAC1 or VDAC2 but not VDAC3 restores rhythmic cardiac contractions in tremblor/ncx1h mutants. (A) Western blot with anti-FLAG antibody after SDS-PAGE with lysate from 32 hpf uninjected embryos or embryos injected with 25 pg. of C-terminally FLAG-tagged VDAC mRNA demonstrates that all VDAC mRNAs are expressed at comparable levels in embryos. β-actin was used as a loading control. (B) While injection of 25 pg. VDAC1 or VDAC2 mRNA significantly restored rhythmic cardiac contractions in 1-day-old tremblor/ncx1h mutant embryos (46.5 ± 3.4%, N = 3, n = 120, and 44.8 ± 0.4%, N = 3, n = 134, respectively, as opposed to 14 ± 3.5%, N = 3, n = 175 in uninjected siblings), injection of 25 pg. VDAC3 failed to recapitulate this effect (19.4 ± 1.1%, N = 3, n = 114). Overall rescue percentages represent the mean rescue percentage ± s.e.m. from N independent experiments, using a total of n embryos.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.