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Figure 2
Three-dimensional imaging analysis helps to orient and localize the OP of zebrafish for TEM studies. (A,B). Immunofluorescent labelling with anti-acetylated α-tubulin shows the distribution of multiciliated cells in the OP of 4 dpf larvae. Software Imaris (Bitplane) v.9.5.0 allowed 3D blend reconstructions of 2 different OPs from 2 different larvae. (C,D) 3D surface reconstructions from the respective OPs revealing the concave morphology of the organ when rotated. Anti-Acetylated α-tubulin immunofluorescence in magenta and DAPI in cyan. Scale bar 20 μm.
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Full text @ Int. J. Mol. Sci.