FIGURE 4

FIGURE 4

Expression and dynamics of eGFP- Smn Δex6,7 in zebrafish motor neurons. (A) Expression of eGFP- SmnΔex6,7 in motor neuron cell body and axon at 2 dpf. It is expressed strongly in the nucleus and weakly in axon and cytoplasm of cell body. (B) Representative ACF and fitted ACF of eGFP, eGFP-Smn and eGFP-SmnΔex6,7. (C) Average diffusion coefficients, D₁ (μm²/s) of eGFP, eGFP-Smn, eGFP-SmnΔex6,7 in cell body. (D) Average diffusion coefficients, D₂ (μm²/s) of eGFP-Smn, eGFP-SmnΔex6,7 in cell body. (E) Average diffusion coefficients, D₁ (μm²/s) of eGFP, eGFP-Smn, eGFP- SmnΔex6,7 in axons. (F) Average diffusion coefficients, D₂ (μm²/s) of eGFP-Smn, eGFP-SmnΔex6,7 in axons. Error bars represent the SD. (^n.s.P > 0.05, ^∗P < 0.05, ^∗∗∗P < 0.001). Measurements grouped under Case 1 were fitted to 3D 1-particle diffusion model, found in 6 out of 14 measurements, indicating a loss of the second component. Case 2 refers to measurements fitted to 3D 2-particle diffusion model, found in 8 out of 14 measurements. The numbers above the graph indicate the numbers of fish (number of points) the measurements were taken in.