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Figure 5
The expression levels of elovl8a and elovl8b in liver of diet-treatment zebrafish and other elongase knockout zebrafish. (A) The expression levels of elovl8a in the liver of C18:3n-3 diet-treatment zebrafish. (B,C) The expression levels of elovl8b in the liver of C18:0 and C20:0 diet-treatment zebrafish. (D) The expression levels of elovl2, elovl4s, and elovl5 in the liver of elovl8a knockout zebrafish (elovl8a−/−). (E) The expression levels of elovl1s, elovl3s, and elovl7s in the liver of elovl8b knockout zebrafish (elovl8b−/−). (F) The expression levels of elovl8a in the liver of elovl5 knockout zebrafish (elovl5−/−). (G–I) The expression levels of elovl8b in the liver of elovl1a knockout zebrafish (elovl1a−/−) (G), elovl1b knockout zebrafish (elovl1b−/−) (H), and elovl3b knockout zebrafish (elovl3b−/−) (I). The statistical analyses were conducted by the t test. Data were expressed as mean ± SD (standard deviation) of three biological replicates. Asterisks above the error bars indicate significant differences (* p < 0.05, ** p < 0.01). WT, wild type zebrafish; elovl, elongation of very long-chain fatty acid protein.