FIGURE 1

FIGURE 1

Expression and dynamics of mCherry and mCherry-Smn in SH-SY5Y cell culture. (A) Expression of mCherry and mCherry-Smn in SH-SY5Y cells 2 days after transfection. Scale bar = 10 μm. (B) Representative autocorrelation function (ACF) and fitted ACFs of mCherry and mCherry-Smn in the cytoplasm. ACF for mCherry is fitted to 3D 1p1t while ACF for mCherry-Smn is fitted to 3D 2p1t diffusion model. (C) Mean diffusion coefficients, D₁ (μm²/s) in cytoplasm and nucleus for mCherry, mCherry-Smn and mCherry-Smn: Smn (mCherry-Smn co-transfected with untagged Smn). (D) Mean diffusion coefficients, D₂ (μm²/s) in cytoplasm and nucleus. Error bars represent the SD. (^∗∗P < 0.01, ^****P < 0.0001). (E) Brightness analysis of mCherry and mCherry-Smn in cytoplasm by plotting average intensity vs. number of particles. mCherry-Smn particles are brighter than mCherry. (F) Brightness analysis of mCherry-Smn vs. mCherry-Smn with untagged Smn in the cytoplasm. With presence of untagged Smn, brightness of mCherry-Smn goes down. The numbers above the graphs indicate the number of cells (number of points) the measurements were taken in.