|
Figure 3
TBI inflicted zebrafish display edema and neuroinflammation. (A) Graph of level of edema, measured as the percentage of fluid in the brain, at all severities from 1?28 dpi, in which edema increased in a severity-dependent fashion, and remained elevated until 3 dpi and 5 dpi for moTBI and sTBI, respectively (n = 9). (B,C) Expression of pro-inflammatory cytokine genes il-1b (B) and tnfa (C) were significantly elevated in sTBI for 3 or 1 dpi, respectively, following injury before returning to near undamaged levels (5 pooled cerebellums/group, n = 3 groups). (D) Anti-inflammatory cytokine il-10 gene expression was also elevated and remained highly upregulated for 14 dpi (5 pooled cerebellums/group, n = 3 groups). Mean ± SEM is depicted in all graphs. Statistical analyses were performed with a One-way ANOVA followed by a Tukey?s or Dunnett?s multiple comparison post-hoc test. # p < 0.05, ## p < 0.01.