Fig. 6

Fig. 6

SRPIN340 treatment perturbs intersegmental vessels (ISV) sprouting and DLAV formation in zebrafish embryos. a The schedule sketch indicates the protocol used for drug treatment of Tg(fli1:EGFP) Casper embryos. b Immunofluorescence image of the vasculature of a Tg(fli1:EGFP) embryo at 42hpf (Left). Box represents the zoom area showing the trunk vasculature (Right). DLAV, dorsal longitudinal anastomic vessel; ISV, intersegmental vessel; DA, dorsal aorta; PCV, posterior cardinal vein. c Confocal images of EGFP⁺ vessels in the trunk of Tg(fli1:EGFP) zebrafish embryos at 28hpf (upper panel) and 42hpf (lower panel) after exposure to DMSO (as vehicle control), SSR or SRPIN 340 at 100μM. Arrows indicate disrupted ISVs. Stars point to incomplete DLAV formation. d Quantification of blood vessel formation defects. (Left) Quantification of ISV length with ImageJ software. Each value corresponds to the mean of length measurement of at least ten ISVs in the trunk of a same embryo. * represents a significant statistical difference between the indicated groups (one-way ANOVA test; ****p<0.0001). (Right) Quantification of DLAV phenotype scored as absent, interrupted, or complete in 42 hpf Tg(fli1:EGFP) embryos treated or not with SSR or SRPIN 340. Values are expressed in percentage of the total embryos analyzed in each condition. n = number of embryos