IMAGE

FIGURE 2

ID

ZDB-IMAGE-210828-9

Antibodies

Ab3-casp3

Publication

Islam et al., 2021 - The Zebrafish Meiotic Cohesin Complex Protein Smc1b Is Required for Key Events in Meiotic Prophase I

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Figures for Islam et al., 2021

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Figure Caption

FIGURE 2

Smc1b mutant zebrafish display defects in spermatogenesis. (A)Smc1b mutations used in this study: smc1b^Q198X located in exon 4 and smc1b^Q261X in exon 5. Both mutations cause premature termination codons. (B–E) Hematoxylin-eosin (H&E) staining on adult testes of wild type (B), smc1b^{Q261X(–/–)}(C), smc1b^{Q198X(–/–)}(D), and smc1b^Q198X/Q261X(E). Dashed lines denote examples of: spermatogonia (yellow), spermatocytes (red), and spermatozoa (orange). (F,G) Caspase-3 (green) and DAPI (blue) staining on adult testes from wild type (F) and smc1b mutant (G). Caspase-3 positive cells are indicated by arrows. (H) Quantification of caspase-3 positive cells per testis area in the mutant (N = 3 testes) and wild type (N = 3 testes). Student’s t-test shows no significant (p = 0.3524) difference between wild-type and mutant caspase-3 positive cells numbers. Scale bars are 50 μm.

Figure Data

Expression / Labeling details

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol