ZFIN Image: Melo et al., 2021, Figure 4

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Figure 4

Analysis of angiogenesis in vivo. This assay was performed with Tg [Fli1: eGFP] embryos (10 embryos per group). (A) the image of zebrafish embryos. Control, untreated embryos. Peptide, embryos treated with the HS-binding peptide. Scramble, embryos treated with scrambled peptide. Assays were performed with zebrafish embryos 2 days after fertilization (2 dpf). After 24 hours of treatment, the animals were analyzed by confocal microscopy and the images obtained in 10X magnification. Subintestinal vessels were analyzed. (B) the quantification of the subintestinal vessels was evaluated using ImageJ software. The values express the mean and standard deviation in each group. *p < 0.05 (ANOVA). The HS-binding peptide was capable of inhibiting angiogenesis of sub intestinal vessels.

Acknowledgments

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