Fig. 6.

Fig. 6.

Functional efficacy of MPDA-DMEA nanoparticles in ML-1 thyroid cancer cells. A–C Confocal images of ML-1 cells with control siRNA-Alexa Fluor 555 loaded nanoparticles. A (DAPI), B (siRNA-Alexa Fluor 555), and C (Merge: DAPI + siRNA-Alexa Fluor 555). The scale bar is 20 μm. The images shown are representative of at least three separate experiments. D Representative western blot showing the efficacy of siSTIM1 in ML-1 cells. E Representative western blot showing the efficacy of siSTIM1-loaded MPDA-DMEA nanoparticles in ML-1 cells. NL-siRNA denotes nanoparticles loaded siRNA. F, G siSTIM1-loaded nanoparticles decreased the invasion and proliferation of ML-1 cells. UnT, un-treated; E.Nano, empty MPDA-DMEA nanoparticles, siC Nano, control non-target siRNA loaded nanoparticles, siSTIM1 Nano, siSTIM1 loaded nanoparticles. *ML-1 unT vs siSTIM1 Nano, ^osiC Nano vs siSTIM1 Nano. The bars show the mean ± S.E. (n = 6). **p < 0.01; ***p < 0.001; ^oop < 0.01; ^ooop < 0.001