Fig. 3

Fig. 3

STIM1 or ORAI1 knock-down attenuates invasion and receptor expression in thyroid cancer ML-1 cells. A, D, STIM1 or ORAI1 knock-down both decreased the basal and abolished the S1P-evoked (final concentration, 100 nm) invasion in ML-1 cells. The bars show the mean ± S.E. (n = 4). *Denotes comparison with MOCK cells. ***p < 0.001. B STIM1 knock-down decreased the relative expression of S1P₃. A representative Western blot is shown. The bar diagram shows the decreased expression of S1P₃ in STIM1-KD cells compared with MOCK cells (mean ± S.E., n = 3) ***p < 0.001. C STIM1 knock-down decreased the relative expression of VEGFR2. A representative Western blot is shown. The bar diagram shows the decreased expression of VEGFR2 in STIM1-KD cells compared with MOCK cells (mean ± S.E., n = 3). ***p < 0.001. E ORAI1 knock-down decreased the relative expression of S1P₁. A representative Western blot is shown. The bar diagram shows the decreased expression of S1P₁ in ORAI1-KD cells compared with MOCK cells (mean ± S.E., n = 3). ***p < 0.001. F ORAI1 knock-down decreased the relative expression of VEGFR2. A representative Western blot is shown. The bar diagram shows the decreased expression of VEGFR2 in STIM1-KD cells compared with MOCK cells (mean ± S.E., n = 3). ***p < 0.001