ZFIN Image: Sehgal et al., 2021, Fig. EV5

Image

Figure Caption

Fig. EV5 Validation of pathophysiology associated to diabetic retinopathy of patient samples

A, B

H&E‐based immunohistochemistry of retina indicating symptoms of diabetic retinopathy (DR). (A) Retina of control sample indicating regular retinal structures with proper cellular organization. (B) Retina samples of DM patients highlighted early symptoms of retinopathy in form of degeneration of ganglion layer, microaneurysm, arteriolar dilatation, and mild edema. Scale bar is 200 μm.

C–F

Retina scan of patients highlighting symptoms of retinopathy. (C) Fundus fluorescence angiography of patients showing vessel integrity defects. (D) Fundus photograph of a patient with symptoms of proliferative diabetic retinopathy and having subhyaloid hemorrhages. (E) Fundus photograph of a patient with symptoms of proliferative diabetic retinopathy with fibrovascular proliferation at disk and abnormal new blood vessels (NVE). (F) Optical coherence tomography of patients with diabetic macular edema.

G

Dot plot representing relative expression of VEAL2 (in fold change) in fibrous membrane isolated from control and PDR patients. Data obtained from 7 patients as biological replicates and represented as individual values with mean fold change values ± standard deviation.

H

Bar graph representing relative expression of VEAL2 in control of HUVECs and hyperglycemia stimulated HUVECs by growing under high glucose. Data are acquired from 3 different biological replicates and shown as individual values with mean fold change values ± standard deviation.

Data information: All the experiments N ≥ 3. ****P‐value < 1E‐4. Statistics: unpaired two‐tailed t‐test.

Acknowledgments

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