ZFIN Image: Sehgal et al., 2021, Fig. EV3

Image

Figure Caption

Fig. EV3 Overexpression and knockdown of <italic toggle='yes'>VEAL2</italic> regulate migration and proliferation in HUVECs

A

Representative images showing wound closure rate in overexpressed VEAL2 and control plasmid‐transfected HUVEC monolayer at 0, 9, and 24 h post‐scratch. Images taken at 10× magnification with scale bar representing 50 μm.

B

Dot plot representing wound closure rate at 0, 9, and 24 h post‐scratch in control cells and veal2‐overexpressed HUVEC monolayer. Data from 3 different technical replicates of 3 biological replicates are presented. Data are plotted as individual values; the middle bar represents the mean, and the error bar represents ± standard deviation.

C

Representative images showing wound closure rate in control siRNA‐ and VEAL2 siRNA‐transfected HUVEC monolayer at 0, 9, and 24 h post‐scratch. Images taken at 10× magnification with scale bar representing 50 μm.

D

Dot plot representing wound closure rate at initial time, 9, and 24 h post‐scratch in control siRNA‐ and VEAL2 siRNA‐transfected HUVEC monolayer. Data from 3 different technical replicates of 3 biological replicates are presented. Data are plotted as individual values; the middle bar represents the mean, and the error bar represents ± standard deviation.

E, F

smFISH of VEAL2 in HUVECs transfected with control siRNA and VEAL2 siRNA shows specificity of cytoplasmic signal of VEAL2. VEAL2 in CAL Fluor Red (610 nM). Merged image for VEAL2 and DAPI. Magnification‐100× and scale bar‐5 μm.

Data information: All the experiments N ≥ 3. **P‐value < 1E‐2 and ***P‐value < 1E‐3. Statistics: one‐way ANOVA with Bonferroni’s multiple data comparison.

Acknowledgments

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