ZFIN Image: Sehgal et al., 2021, Fig. 2

Image

Figure Caption

Fig. 2 TALEN‐based gene editing of <italic toggle='yes'>veal2</italic> locus caused vascular integrity defects in <italic toggle='yes'>gib004Tg(fli1a:EGFP;gata1a:DsRed)</italic> zebrafish embryos

A

Schematic representation of TALEN design, its injection into one‐cell zebrafish embryos, and screening at 2 dpf for any phenotypic changes.

B

List of sequences showing indels at the veal2 locus at somatic level in F₀ zebrafish embryos injected with TALEN arms.

C

Schematic representation of outcross of veal2 heterozygous mutant veal2^gib005Δ8/+ and the chromatograms representing the two different types of alleles identified on genotyping of 20 embryos randomly.

D

Bar graph representing the number of animals which displayed the hemorrhage phenotype across the progeny derived from breeding of two control gib004Tg(fli1a:EGFP;gata1a:DsRed) zebrafish and progeny derived from an outcross of veal2^gib005Δ8/+ zebrafish. Data from three different experiments plotted as mean percentage values ± standard deviation.

E

Bar graph representing the number of animals which displayed the hemorrhage phenotype across the progeny derived from an outcross of veal2^gib005Δ8/+ zebrafish injected with veal2 RNA and vehicle control separately. Data from three different experiments plotted as mean percentage values ± standard deviation.

F–T

Representative images of 2 dpf zebrafish which displayed the rescue of the vascular integrity defects in the progeny of an outcross of veal2^gib005Δ8/+ zebrafish upon complementing with the wild‐type (WT) veal2 RNA. (F–J) Control zebrafish embryos. (K–O) veal2^gib005Δ8/+ embryos injected with vehicle control. (P–T) veal2^gib005Δ8/+ embryos complemented with veal2 RNA. (F, K, P) Bright field. (G, L, Q) mRFP. (H, M, R) Animals stained with O‐dianisidine stain. (I, N, S) eGFP. (J, O, T) Merged eGFP and mRFP filters. Arrowheads show the presence of hemorrhage due to the vascular integrity defects. (F–H, K–M, P–R) Magnification‐5× and scale bar‐100 μm. (I–J, N–O, S–T) Magnification‐20× and scale bar‐50 μm.

Data information: All the experiments N ≥ 3. ****P‐value < 1E‐4 and ***P‐value = 1E‐4. Statistics‐unpaired two‐tailed t‐tests.

Source data are available online for this figure.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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