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Fig. 4.

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ZDB-IMAGE-210814-58
Source
Figures for Cardenas-Rodriguez et al., 2021
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Figure Caption

Fig. 4.

MZcep290−/− mutants present milder phenotypes that recover after 4-5 dpf. (A) Clutch of cep290 maternal zygotic cep290 mutants. Body shape curvature seen at 3 dpf was lost at 7 dpf (n=100). (B) Maternal zygotic cep290 mutants reach adulthood and can present scoliosis (15-40% of adult fish; n=31). (C) RT-PCR from MZcep290−/− and wild-type embryos. No exon skipping around exon 16 was detected in mutant embryos. (D) RT-qPCR from adult eye and kidney samples (n=3). Specific wild-type (WT) and mutant primers for the targeted exon 16 were used to quantify wild-type and mutated cep290 mRNA. As expected, wild-type primers did not generate an RT-PCR product with mutant mRNA samples relative to wild-type mRNA, whereas mutant primers generated significant product with mutant mRNA samples relative to wild-type mRNA samples. Primers for ex3-ex5, ex19-ex20 and ex50-52 were used to quantify nonsense-mediated decay. No changes in cep290 mRNA levels were detected in MZcep290−/− mutant samples compared with controls. n=3 biological replicates. Data are mean±s.d. Experiments were replicated at a minimum three times. *P<0.05; ****P<0.0001; ns, not significant (unpaired t-test).

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.