Behavioral effects of the crude methanolic extract, EA, and 50% MeOH fractions of Clitorea ternatea roots on the reserpine-induced stress zebrafish model. Behavioral measurements were recorded at (A) 24 h after IP injection of reserpine, (B) 24 h after treatments, (C) 48 h after treatments, and (D) 72 h after treatments for each group, i.e., fluoxetine (0.6 mg/mL, 30 min), CTRE, EA, and 50% MeOH fractions groups (35 mg/mL, 6 h). All values are reported as mean percentage ± SE (n = 10 per group). Duncan’s post-hoc test was used for significant differences, where * = p < 0.05, ** = p < 0.01, and *** = p < 0.001. PBS: phosphate buffer saline; FX: fluoxetine; RES: reserpine; CTRE: crude C. ternatea extract; EA: ethyl acetate; MEOH: 50% methanol.
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