Examination of the associations of TcpAh with TRAF3 and TBK1. (A) Co-localization analysis of TcpAh and zebrafish TRIF protein in HEK293T cells by confocal microscopy (Zeiss LSM 710; original magnification, 630×). The nucleus was stained with DAPI. Scale bars correspond to 10 μm. (B) Co-IP analysis between TcpAh and zebrafish TRIF proteins from HEK293T cells expressing Myc-TcpAh with Flag-TRIF. (C) Co-localization analysis of TcpAh and zebrafish TRAF3 or TBK1 proteins in HEK293T cells by confocal microscopy (Zeiss LSM 710; original magnification, 630×). The nucleus was stained with DAPI. Scale bars correspond to 10 μm. (D) Co-IP analysis between TcpAh and zebrafish TRAF3 or TBK1 proteins from HEK293T cells expressing Myc-TcpAh with Flag-TRAF3 or Flag-TBK1.
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