FIGURE 4

FIGURE 4

Interaction between TcpAh and MyD88 by TIR–TIR and TIR–DD interactions. (A) Co-localization analysis of TcpAh and zebrafish MyD88 proteins in HEK293T cells by confocal microscopy (Zeiss LSM 710; original magnification, 630×). The nucleus was stained with DAPI. Scale bars correspond to 10 μm. (B) Co-IP analysis between TcpAh and zebrafish MyD88 proteins from HEK293T cells expressing Myc-TcpAh with GFP or GFP-MyD88. (C) Schematic diagram of wild-type and domain-truncated zebrafish MyD88 forms. (D) Co-localization analysis between TcpAh and truncated MyD88 proteins in HEK293T cells by confocal microscopy (Zeiss LSM 710; original magnification, 630×). The nucleus was stained with DAPI. Scale bars correspond to 10 μm. (E) Co-IP assay between TcpAh and MyD88-TIR domain (148–284 aa) or MyD88-DD domain (11–101 aa) as shown in (B), except MyD88-TIR or MyD88-DD was used instead of full-length wild-type MyD88.