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Figure 7 MiR-145 upregulation affects the expression of NCC marker genes. (a?h) Whole-mount in situ hybridization of sox9a (a,b,e,f) and sox9b (c,d,g,h) performed on 13 hpf miR-145-dsRED microinjected embryos. Lateral views are dorsal to top (a,e,c,g); dorsal views are anterior to left (b,f,d,h). In (a) ov: otic vesicle; s: somites. In (c) d: diencephalon; ov: otic vesicle; nc: neural crest. Scale bar represents 200 ?m in (a) for (a?h). (i?r) Whole-mount in situ hybridization of col2a1a (ENSDARG00000069093) in miR-145-dsRED microinjected specimens at 24 hpf (i,n), 48 hpf (j,k,o,p), and 72 hpf (l,m,q,r). Lateral and dorsal views are head to left. In (k) ch: ceratohyal; pc: parachordals. In (l,m) et: ethmoid plate; tr: trabeculae. (s) Schematic representation of col2a1a 3?UTR. Putative miRNA binding sites are shown below each region. MiR-145 site is enclosed in a purple box, with its respective context+ score. Scale bars represent 200 ?m in (i) for (i,n); and in (j) for (j?m) and (o?r).